LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2405..2501) /label=attL2 /note="attL2 (c)" misc_signal complement(2516..2535) /note="T7 Promoter/priming site (c)" primer_bind 2543..2559 /note="M13 Reverse priming site" gene 2672..3481 /note="Kanamycin resistance gene" rep_origin 3602..4275 /note="pUC origin" vector join(2413..4278,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2398 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:104846 IMAGE:6672104" /tissue_type="Ovary, teratocarcinoma" /clone_lib="NIH_MGC_109" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2398 /gene="CERCAM" /gene_synonym=GLT25D3 /db_xref="GeneID:51148" /db_xref="HGNC:23723" CDS 860..2398 /dnas_title="cerebral endothelial cell adhesion molecule" /gene="CERCAM" /gene_synonym=GLT25D3 /codon_start=1 /product="cerebral endothelial cell adhesion molecule" /protein_id="AAH98432.2" /db_xref="GI:127802779" /db_xref="GeneID:51148" /db_xref="HGNC:23723" /translation="MLQEWLAAVGDDYAAVVWRPEGEPRFYPDEEGPKHWTKERHQFL MELKQEALTFARNWGADYILFADTDNILTNNQTLRLLMGQGLPVVAPMLDSQTYYSNF WCGITPQGYYRRTAEYFPTKNRQRRGCFRVPMVHSTFLASLRAEGADQLAFYPPHPNY TWPFDDIIVFAYACQAAGVSVHVCNEHRYGYMNVPVKSHQGLEDERVNFIHLILEALV DGPRMQASAHVTRPSKRPSKIGFDEVFVISLARRPDRRERMLASLWEMEISGRVVDAV DGWMLNSSAIRNLGVDLLPGYQDPYSGRTLTKGEVGCFLSHYSIWEEVVARGLARVLV FEDDVRFESNFRGRLERLMEDVEAEKLSWDLIYLGRKQVNPEKETAVEGLPGLVVAGY SYWTLAYALRLAGARKLLASQPLRRMLPVDEFLPIMFDQHPNEQYKAHFWPRDLVAFS AQPLLAAPTHYAGDAEWLSDTETSSPWDDDSGRLISWSGSQKTLRSPRLDLTGSSGHS LQPQPRDEL" misc_feature 692..2398 /note="CERCAM coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgcgggcgtt gcggagtcgc cgctgcccgc 721 cgtggtcctt gccatcctgg cccgcaatgc cgaacactcg ctgccccact acctgggcgc 781 tctggagcgg ctggactacc cccgggccag gatggccctc tggtgtgcca cggaccacaa 841 tgtggacaac accacagaga tgctgcagga gtggctggcg gctgtgggcg atgactatgc 901 tgctgtggtc tggaggcctg agggcgagcc caggttctac ccagatgaag agggtcccaa 961 gcactggacc aaagaaaggc accagtttct gatggagctg aagcaggaag ccctcacctt 1021 tgccaggaac tggggggccg actatatcct gtttgcagac acagacaaca ttctgaccaa 1081 caatcagact ctgcggcttc tcatggggca ggggcttcca gtggtggccc caatgctgga 1141 ctcccagacc tactactcca acttctggtg tgggatcacc ccccagggct actaccgccg 1201 cacagccgag tacttcccca ccaagaaccg ccagcgccgg ggctgcttcc gtgtccccat 1261 ggtccactcc accttccttg catccctgcg ggctgaaggg gcagaccagc ttgctttcta 1321 cccgccacat cccaactaca cttggccttt cgacgacatc atcgtcttcg cctatgcctg 1381 ccaggctgct ggggtctccg tccacgtgtg caatgagcac cgttatgggt acatgaatgt 1441 gccggtgaaa tcccaccagg ggctggaaga cgagagggtc aacttcatcc acctgatctt 1501 agaagcacta gtggacggcc cccgcatgca ggcctcagct catgtgactc ggccctctaa 1561 gaggcccagc aagatagggt ttgacgaggt ctttgtcatc agcctggctc gcaggcctga 1621 ccgtcgggaa cgcatgctcg cctcgctctg ggagatggag atctctggga gggtggtgga 1681 cgctgtggat ggctggatgc tcaacagcag tgccatcagg aacctcggcg tagacctgct 1741 cccgggctac caggaccctt actcgggccg cactctgacc aagggcgagg tgggctgctt 1801 cctcagccat tactccatct gggaagaggt ggttgccagg ggcctggccc gggtcctggt 1861 gtttgaggat gacgtgcgct ttgagagcaa cttcaggggg cggctggagc ggctgatgga 1921 ggatgtggag gcagagaaac tgtcttggga cctgatctac ctcggacgga agcaggtgaa 1981 ccctgagaag gagacggccg tggaggggct gccgggcctg gtggtggctg ggtactccta 2041 ctggacgctg gcctatgccc tgcgtctggc gggtgcccgc aagctgctgg cctcacagcc 2101 tctgcgccgc atgctgcccg tggacgagtt cctgcccatc atgttcgacc agcaccccaa 2161 cgagcagtac aaggcacact tctggccacg ggacctggtg gccttctccg cccagcccct 2221 gctcgctgcc cctacccact atgccgggga cgccgagtgg ctcagtgaca cggagacatc 2281 ctctccatgg gatgatgaca gcggccgcct catcagctgg agcggctccc aaaagaccct 2341 gcgcagcccc cgcctggacc tgactggcag cagcgggcac agcctccaac cccagcccTA 2401 GGACCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG 2461 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC 2521 CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA 2581 AAATCTCTGA TGTTACATTG CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC 2641 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG 2701 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA 2761 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT 2821 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT 2881 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA 2941 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA 3001 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA 3061 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC 3121 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG 3181 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC 3241 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT 3301 AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT 3361 ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG 3421 TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA 3481 ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG 3541 CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA 3601 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 3661 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 3721 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT 3781 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 3841 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 3901 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 3961 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 4021 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 4081 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 4141 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 4201 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 4261 GCCTTTTGCT CACATGTT //